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KMID : 0606920070150020073
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Biomolecules & Therapeutics
2007 Volume.15 No. 2 p.73 ~ p.77
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N,N-Dimethyl-D-ribo-phytosphingosine Modulates Cellular Functions of 1321N1 Astrocytes
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Lee Yun-Kyung
Kim Hyo-Lim
Kim Kye-Ok
Sacket Santosh J
Han Mi-Jin
Jo Ji-Yeong
Lim Sung-Mee
Im Dong-Soon
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Abstract
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N,N-Dimethyl-D-ribo-phytosphingosine (DMPH) is an N-methyl derivative of sphingosine. In the present paper, we studied effects of DMPH on intracellular Ca$^{2+}$ concentration, pH, glutamate uptake, and cell viability in human 1321N1 astrocytes. DMPH increased intracellular Ca$^{2+}$ concentration and cytosolic pH significantly in a dose-dependent manner. DMPH also inhibited glutamate uptake by 1321N1 astrocytes. Finally, treatment of cells with DMPH for 24 h reduced viability of cells largely and concentration-dependently. In summary, DMPH increased intracellular Ca$^{2+}$ concentration and pH, inhibited glutamate uptake and evoked cytotoxicity in 1321N1 astrocytes. Our observations with DMPH in the 1321N1 astrocytes would enhance understanding of DMPH actions in the brain.
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KEYWORD
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Sphingosine, Calcium, pH, Glutamate, Astrocytes
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